| Summary: | <table style="height: 467px;" border="0" cellspacing="0" cellpadding="0" width="498" align="left"><tbody><tr><td height="467" align="left" valign="top"><p>The purpose of this study was to determine <em>in vitro</em> antibacterial activity of selected teas (Assam: Indian black tea from <em>Camellia sinensis</em>, Pu-erh: darkpu-erh (shu) from <em>Camellia sinensis</em>, Sencha: Japanese green tea from <em>Camellia sinensis</em>) against five species of pathogenic microorganisms. In our study, we determined the total viable count (TVC), number of yeasts (Y) and number of <em>Enterobacteriaceae</em> genera (E). Then MALDI-TOF MS Biotyper identified colonies after cultivation. Evaluation of the antimicrobial activity was performed by disc diffusion method, well diffusion method and detection of minimum inhibitory concentration (MIC). For antibacterial activity, bacteria <em>Escherichia coli </em>CCM 2024<em>, Yersinia enterocolitica </em>CCM 5671<em>, Klebsiella pneumonie </em>CCM 2318<em>, Staphylococus aureus </em>CCM 2461 and<em> Bacillus thurigiensis </em>CCM19 were used. The inhibition zones were measured in mm in disc diffusion method and well diffusion method. The MIC of the individual extracts was measured spectrophotometrically. The high number of total viable count was found in Pu-erh tea (2.1 log CFU.g<sup>-1</sup>) and lowest number was found in Assam tea (0.7 log CFU.g<sup>-1</sup>). The high number of <em>Enterobacteriacea</em> genera was found in Pu-erh tea (2.03 log CFU.g<sup>-1</sup>) and lowest number was found in Assam tea <br /> (0 log CFU.g<sup>-1</sup>). The higher number of yeasts was found in Pu-erh tea (1.83 log CFU.g<sup>-1</sup>) and lowest number was found in Assam tea (0.3 log CFU.g<sup>-1</sup>). Mass spectrometry revealed seven Gram positive bacteria <em>Bacillus cereus, B. mycoides, B. pumilus, Enterococcus durans, Staphylococcus epidermis, S. hominis, S. warneri</em>, four Gram negative bacteria <em>Acinetobacter junii, Hafnia alvei, Klebsiella pneumoniae, Sphingomonas </em>spp. and two yeast <em>Candida glabrata</em>,<em> Cryptococcus albidus</em>. The results show that certain tea extracts are particularly active against various pathogenic bacteria. Tea extracts (Sencha, Rooibos, Mate, Assam) were found to have the strongest antibacterial activity against bacteria <em>Staphylococcus aureus</em> CCM 2461.<strong></strong></p></td></tr></tbody></table> <!--[endif] -->
|
|---|
