Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing

Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing

Summary: CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on target at the edited locus and off target at other genomic loci. Here, we investigate the occurrence of deleterious on-target effects (OnTEs) in human stem cells after inse...

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Bibliographic Details
Main Authors: Isabel Weisheit, Joseph A. Kroeger, Rainer Malik, Julien Klimmt, Dennis Crusius, Angelika Dannert, Martin Dichgans, Dominik Paquet
Format: Article
Language:English
Published: Elsevier 2020-05-01
Series:Cell Reports
Subjects:
CRISPR
induced pluripotent stem cells
iPSCs
homology-directed repair
HDR
on-target effects
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124720306422

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http://www.sciencedirect.com/science/article/pii/S2211124720306422

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