Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition

Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition

Protein-protein interactions are fundamental to the regulation of protein activity and cellular phyisology. Here the authors present Split Intein-Mediated Protein Ligation, which uses bait and prey proteins fused to intein fragments to generate single intact proteins upon interaction.

Bibliographic Details
Main Authors: Zhong Yao, Farzaneh Aboualizadeh, Jason Kroll, Indira Akula, Jamie Snider, Anna Lyakisheva, Priscilla Tang, Max Kotlyar, Igor Jurisica, Mike Boxem, Igor Stagljar
Format: Article
Language:English
Published: Nature Publishing Group 2020-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-16299-1

Internet

https://doi.org/10.1038/s41467-020-16299-1

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