A robust method for particulate detection of a genetic tag for 3D electron microscopy

A robust method for particulate detection of a genetic tag for 3D electron microscopy

Genetic tags allow rapid localization of tagged proteins in cells and tissues. APEX, an ascorbate peroxidase, has proven to be one of the most versatile and robust genetic tags for ultrastructural localization by electron microscopy (EM). Here, we describe a simple method, APEX-Gold, which converts...

Full description

Bibliographic Details
Main Authors: James Rae, Charles Ferguson, Nicholas Ariotti, Richard I Webb, Han-Hao Cheng, James L Mead, James D Riches, Dominic JB Hunter, Nick Martel, Joanne Baltos, Arthur Christopoulos, Nicole S Bryce, Maria Lastra Cagigas, Sachini Fonseka, Marcel E Sayre, Edna C Hardeman, Peter W Gunning, Yann Gambin, Thomas E Hall, Robert G Parton
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-04-01
Series:eLife
Subjects:
APEX
electron microscopy
particulate marker
3D-electron microscopy
genetically encoded
Online Access:https://elifesciences.org/articles/64630

Internet

https://elifesciences.org/articles/64630

Similar Items