Atorvastatin increases intestinal expression of NPC1L1 in hyperlipidemic men
Inhibition of cholesterol synthesis by 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoAR) inhibitors has been associated with an increase in intestinal cholesterol absorption. This study examined how HMG-CoAR inhibition by atorvastatin modulates expression of key genes involved in intestinal choles...
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doaj-e0fff4ce37b64fb288719a8b97956b602021-04-28T06:03:46ZengElsevierJournal of Lipid Research0022-22752011-03-01523558565Atorvastatin increases intestinal expression of NPC1L1 in hyperlipidemic menAndré J. Tremblay0Benoît Lamarche1Valéry Lemelin2Lizbeth Hoos3Suzanne Benjannet4Nabil G. Seidah5Jr. Harry R. Davis6Patrick Couture7Lipid Research Centre, Laval University, Quebec City, Quebec, Canada; CHUL Research Centre, Institute of Nutraceuticals and Functional Foods, Laval University, Quebec City, Quebec, CanadaLipid Research Centre, Laval University, Quebec City, Quebec, CanadaDepartment of Gastroenterology, CHUL, Laval University, Quebec City, Quebec, CanadaDepartment of Cardiovascular Disease, Merck Research Labs, Kenilworth, NJLaboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, CanadaLaboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, CanadaDepartment of Cardiovascular Disease, Merck Research Labs, Kenilworth, NJTo whom correspondence should be addressed. email: patrick.couture@crchul.ulaval.ca; Lipid Research Centre, Laval University, Quebec City, Quebec, Canada; CHUL Research Centre, Institute of Nutraceuticals and Functional Foods, Laval University, Quebec City, Quebec, CanadaInhibition of cholesterol synthesis by 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoAR) inhibitors has been associated with an increase in intestinal cholesterol absorption. This study examined how HMG-CoAR inhibition by atorvastatin modulates expression of key genes involved in intestinal cholesterol metabolism. A crossover study was conducted in which 22 hyperlipidemic men received atorvastatin, 40 mg/day, or placebo, each for 12 weeks. Gene expression was assessed by real-time PCR using duodenal biopsy samples obtained at the end of each phase of treatment. Treatment with atorvastatin was associated with a 76% reduction in lathosterol and significant increases in sitosterol (70%). Atorvastatin significantly increased intestinal mRNA levels of HMG-CoAR (59%), LDL receptor (LDLR) (52%), PCSK9 (187%), SREBP-2 (44%), and HNF-4α (13%). Furthermore, atorvastatin significantly increased intestinal mRNA levels of NPC1L1 by 19% and decreased mRNA levels of both ABCG5 and ABCG8 by 14%. Positive correlations were observed between changes in SREBP-2 and HNF-4α expression and concurrent changes in the intestinal mRNA levels of HMG-CoAR, LDLR, and NPC1L1. These results indicate that HMG-CoAR inhibition with atorvastatin stimulates the intestinal expression of NPC1L1, LDLR, and PCSK9; increases cholesterol absorption; and reduces expression of ABCG5/8; these effects are most likely mediated by upregulation of the transcription factors SREBP-2 and HNF-4α.http://www.sciencedirect.com/science/article/pii/S0022227520409368Niemann-Pick C1-like 1cholesterol metabolism3-hydroxy-3-methylglutaryl CoA reductaseintestinelow density lipoprotein receptor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
André J. Tremblay Benoît Lamarche Valéry Lemelin Lizbeth Hoos Suzanne Benjannet Nabil G. Seidah Jr. Harry R. Davis Patrick Couture |
spellingShingle |
André J. Tremblay Benoît Lamarche Valéry Lemelin Lizbeth Hoos Suzanne Benjannet Nabil G. Seidah Jr. Harry R. Davis Patrick Couture Atorvastatin increases intestinal expression of NPC1L1 in hyperlipidemic men Journal of Lipid Research Niemann-Pick C1-like 1 cholesterol metabolism 3-hydroxy-3-methylglutaryl CoA reductase intestine low density lipoprotein receptor |
author_facet |
André J. Tremblay Benoît Lamarche Valéry Lemelin Lizbeth Hoos Suzanne Benjannet Nabil G. Seidah Jr. Harry R. Davis Patrick Couture |
author_sort |
André J. Tremblay |
title |
Atorvastatin increases intestinal expression of NPC1L1 in hyperlipidemic men |
title_short |
Atorvastatin increases intestinal expression of NPC1L1 in hyperlipidemic men |
title_full |
Atorvastatin increases intestinal expression of NPC1L1 in hyperlipidemic men |
title_fullStr |
Atorvastatin increases intestinal expression of NPC1L1 in hyperlipidemic men |
title_full_unstemmed |
Atorvastatin increases intestinal expression of NPC1L1 in hyperlipidemic men |
title_sort |
atorvastatin increases intestinal expression of npc1l1 in hyperlipidemic men |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2011-03-01 |
description |
Inhibition of cholesterol synthesis by 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoAR) inhibitors has been associated with an increase in intestinal cholesterol absorption. This study examined how HMG-CoAR inhibition by atorvastatin modulates expression of key genes involved in intestinal cholesterol metabolism. A crossover study was conducted in which 22 hyperlipidemic men received atorvastatin, 40 mg/day, or placebo, each for 12 weeks. Gene expression was assessed by real-time PCR using duodenal biopsy samples obtained at the end of each phase of treatment. Treatment with atorvastatin was associated with a 76% reduction in lathosterol and significant increases in sitosterol (70%). Atorvastatin significantly increased intestinal mRNA levels of HMG-CoAR (59%), LDL receptor (LDLR) (52%), PCSK9 (187%), SREBP-2 (44%), and HNF-4α (13%). Furthermore, atorvastatin significantly increased intestinal mRNA levels of NPC1L1 by 19% and decreased mRNA levels of both ABCG5 and ABCG8 by 14%. Positive correlations were observed between changes in SREBP-2 and HNF-4α expression and concurrent changes in the intestinal mRNA levels of HMG-CoAR, LDLR, and NPC1L1. These results indicate that HMG-CoAR inhibition with atorvastatin stimulates the intestinal expression of NPC1L1, LDLR, and PCSK9; increases cholesterol absorption; and reduces expression of ABCG5/8; these effects are most likely mediated by upregulation of the transcription factors SREBP-2 and HNF-4α. |
topic |
Niemann-Pick C1-like 1 cholesterol metabolism 3-hydroxy-3-methylglutaryl CoA reductase intestine low density lipoprotein receptor |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520409368 |
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