Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release
Gliomas arise in the glial cells of the brain or spine and are the most prevalent and devastating type of brain tumors. Studies of tumor immunology have established the importance of the tumor micro-environment as a driver of oncogenesis. Inflammatory mediators such as IL-1β and IL-18 released by mo...
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doaj-e3523a64d10249f999bc63a1d35123c72020-11-24T22:19:08ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2019-05-01910.3389/fonc.2019.00364430764Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 ReleaseLei Tong0Chuncheng Xie1Yafen Wei2Yunyue Qu3Hongsheng Liang4Yiwei Zhang5Tianye Xu6Xin Qian7Huijia Qiu8Haoyu Deng9Department of Microbiology, Harbin Medical University, Harbin, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Neurology, The Provincal Hospital of Heilongjiang Province, Harbin, ChinaDepartment of Microbiology, Harbin Medical University, Harbin, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Vascular Surgery, RenJi Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Vascular Surgery, RenJi Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaGliomas arise in the glial cells of the brain or spine and are the most prevalent and devastating type of brain tumors. Studies of tumor immunology have established the importance of the tumor micro-environment as a driver of oncogenesis. Inflammatory mediators such as IL-1β and IL-18 released by monocytes regulate transcriptional networks that are required for malignant cell growth. Berberine is a natural botanical alkaloid that is widely found in the Berberis species. Although it has been widely used as an anti-diarrheal treatment in North America for several decades, our study is the first to investigate berberine as an anti-tumor agent in glioma cells. In this study, we demonstrate that berberine significantly inhibits inflammatory cytokine Caspase-1 activation via ERK1/2 signaling and subsequent production of IL-1β and IL-18 by glioma cells. Moreover, we found that berberine treatment led to decreased motility and subsequently cell death in U251 and U87 cells. In addition, our study is the first to indicate that berberine can reverse the process of epithelial-mesenchymal transition, a marker of tumor invasion. Taken together, our work supports berberine as a putative anti-tumor agent targeting glioma cells.https://www.frontiersin.org/article/10.3389/fonc.2019.00364/fullgliomasberberineEMTERK1/2IL-1βIL-18 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lei Tong Chuncheng Xie Yafen Wei Yunyue Qu Hongsheng Liang Yiwei Zhang Tianye Xu Xin Qian Huijia Qiu Haoyu Deng |
spellingShingle |
Lei Tong Chuncheng Xie Yafen Wei Yunyue Qu Hongsheng Liang Yiwei Zhang Tianye Xu Xin Qian Huijia Qiu Haoyu Deng Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release Frontiers in Oncology gliomas berberine EMT ERK1/2 IL-1β IL-18 |
author_facet |
Lei Tong Chuncheng Xie Yafen Wei Yunyue Qu Hongsheng Liang Yiwei Zhang Tianye Xu Xin Qian Huijia Qiu Haoyu Deng |
author_sort |
Lei Tong |
title |
Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release |
title_short |
Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release |
title_full |
Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release |
title_fullStr |
Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release |
title_full_unstemmed |
Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release |
title_sort |
antitumor effects of berberine on gliomas via inactivation of caspase-1-mediated il-1β and il-18 release |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2019-05-01 |
description |
Gliomas arise in the glial cells of the brain or spine and are the most prevalent and devastating type of brain tumors. Studies of tumor immunology have established the importance of the tumor micro-environment as a driver of oncogenesis. Inflammatory mediators such as IL-1β and IL-18 released by monocytes regulate transcriptional networks that are required for malignant cell growth. Berberine is a natural botanical alkaloid that is widely found in the Berberis species. Although it has been widely used as an anti-diarrheal treatment in North America for several decades, our study is the first to investigate berberine as an anti-tumor agent in glioma cells. In this study, we demonstrate that berberine significantly inhibits inflammatory cytokine Caspase-1 activation via ERK1/2 signaling and subsequent production of IL-1β and IL-18 by glioma cells. Moreover, we found that berberine treatment led to decreased motility and subsequently cell death in U251 and U87 cells. In addition, our study is the first to indicate that berberine can reverse the process of epithelial-mesenchymal transition, a marker of tumor invasion. Taken together, our work supports berberine as a putative anti-tumor agent targeting glioma cells. |
topic |
gliomas berberine EMT ERK1/2 IL-1β IL-18 |
url |
https://www.frontiersin.org/article/10.3389/fonc.2019.00364/full |
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