Nanobody-enabled monitoring of kappa opioid receptor states
Recent studies revealed that G protein-coupled receptors rapidly interconvert between multiple states. Here, authors use the kappa opioid receptor (KOR) and show how two state-dependent nanobodies provide real-time reporting of ligand stabilized states with KOR and other GPCRs.
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2020-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-14889-7 |
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doaj-ea2f79e5cad94be58517658c2739cb152021-05-11T09:14:09ZengNature Publishing GroupNature Communications2041-17232020-03-0111111210.1038/s41467-020-14889-7Nanobody-enabled monitoring of kappa opioid receptor statesTao Che0Justin English1Brian E. Krumm2Kuglae Kim3Els Pardon4Reid H. J. Olsen5Sheng Wang6Shicheng Zhang7Jeffrey F. Diberto8Noah Sciaky9F. Ivy Carroll10Jan Steyaert11Daniel Wacker12Bryan L. Roth13Department of Pharmacology, University of North Carolina at Chapel Hill School of MedicineDepartment of Pharmacology, University of North Carolina at Chapel Hill School of MedicineDepartment of Pharmacology, University of North Carolina at Chapel Hill School of MedicineDepartment of Pharmacology, University of North Carolina at Chapel Hill School of MedicineStructural Biology Brussels, Vrije Universiteit Brussel (VUB)Department of Pharmacology, University of North Carolina at Chapel Hill School of MedicineDepartment of Pharmacology, University of North Carolina at Chapel Hill School of MedicineDepartment of Pharmacology, University of North Carolina at Chapel Hill School of MedicineDepartment of Pharmacology, University of North Carolina at Chapel Hill School of MedicineDepartment of Pharmacology, University of North Carolina at Chapel Hill School of MedicineResearch Triangle Institute, Research Triangle ParkStructural Biology Brussels, Vrije Universiteit Brussel (VUB)Department of Pharmacology, University of North Carolina at Chapel Hill School of MedicineDepartment of Pharmacology, University of North Carolina at Chapel Hill School of MedicineRecent studies revealed that G protein-coupled receptors rapidly interconvert between multiple states. Here, authors use the kappa opioid receptor (KOR) and show how two state-dependent nanobodies provide real-time reporting of ligand stabilized states with KOR and other GPCRs.https://doi.org/10.1038/s41467-020-14889-7 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Tao Che Justin English Brian E. Krumm Kuglae Kim Els Pardon Reid H. J. Olsen Sheng Wang Shicheng Zhang Jeffrey F. Diberto Noah Sciaky F. Ivy Carroll Jan Steyaert Daniel Wacker Bryan L. Roth |
| spellingShingle |
Tao Che Justin English Brian E. Krumm Kuglae Kim Els Pardon Reid H. J. Olsen Sheng Wang Shicheng Zhang Jeffrey F. Diberto Noah Sciaky F. Ivy Carroll Jan Steyaert Daniel Wacker Bryan L. Roth Nanobody-enabled monitoring of kappa opioid receptor states Nature Communications |
| author_facet |
Tao Che Justin English Brian E. Krumm Kuglae Kim Els Pardon Reid H. J. Olsen Sheng Wang Shicheng Zhang Jeffrey F. Diberto Noah Sciaky F. Ivy Carroll Jan Steyaert Daniel Wacker Bryan L. Roth |
| author_sort |
Tao Che |
| title |
Nanobody-enabled monitoring of kappa opioid receptor states |
| title_short |
Nanobody-enabled monitoring of kappa opioid receptor states |
| title_full |
Nanobody-enabled monitoring of kappa opioid receptor states |
| title_fullStr |
Nanobody-enabled monitoring of kappa opioid receptor states |
| title_full_unstemmed |
Nanobody-enabled monitoring of kappa opioid receptor states |
| title_sort |
nanobody-enabled monitoring of kappa opioid receptor states |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2020-03-01 |
| description |
Recent studies revealed that G protein-coupled receptors rapidly interconvert between multiple states. Here, authors use the kappa opioid receptor (KOR) and show how two state-dependent nanobodies provide real-time reporting of ligand stabilized states with KOR and other GPCRs. |
| url |
https://doi.org/10.1038/s41467-020-14889-7 |
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1721450157725188096 |