Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii
Cronobacter sakazakii is a foodborne pathogen that can cause a rare, septicemia, life-threatening meningitis, and necrotizing enterocolitis in infants. In general, standard methods for pathogen detection rely on culture, plating, colony counting and polymerase chain reaction DNA-sequencing for ident...
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2018-06-01
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doaj-eb4539330bda49cfaa71a197f200fcda2020-11-25T02:31:01ZengMDPI AGSensors1424-82202018-06-01187202810.3390/s18072028s18072028Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakiiMohamed A. Aly0Konrad J. Domig1Wolfgang Kneifel2Erik Reimhult3Department of Nanobiotechnology, Institute for Biologically Inspired Materials, BOKU—University of Natural Resources and Life Sciences, Vienna, Vienna A-1190, AustriaDepartment of Food Science and Technology, Institute of Food Science, BOKU—University of Natural Resources and Life Sciences, Vienna, Vienna A-1190, AustriaDepartment of Food Science and Technology, Institute of Food Science, BOKU—University of Natural Resources and Life Sciences, Vienna, Vienna A-1190, AustriaDepartment of Nanobiotechnology, Institute for Biologically Inspired Materials, BOKU—University of Natural Resources and Life Sciences, Vienna, Vienna A-1190, AustriaCronobacter sakazakii is a foodborne pathogen that can cause a rare, septicemia, life-threatening meningitis, and necrotizing enterocolitis in infants. In general, standard methods for pathogen detection rely on culture, plating, colony counting and polymerase chain reaction DNA-sequencing for identification, which are time, equipment and skill demanding. Recently, nanoparticle- and surface-based immunoassays have increasingly been explored for pathogen detection. We investigate the functionalization of gold nanoparticles optimized for irreversible and specific binding to C. sakazakii and their use for spectroscopic detection of the pathogen. We demonstrate how 40-nm gold nanoparticles grafted with a poly(ethylene glycol) brush and functionalized with polyclonal antibodies raised against C. sakazakii can be used to specifically target C. sakazakii. The strong extinction peak of the Au nanoparticle plasmon polariton resonance in the optical range is used as a label for detection of the pathogens. Individual binding of the nanoparticles to the C. sakazakii surface is also verified by transmission electron microscopy. We show that a high degree of surface functionalization with anti-C. sakazakii optimizes the detection and leads to a detection limit as low as 10 CFU/mL within 2 h using a simple cuvette-based UV-Vis spectrometric readout that has great potential for further optimization.http://www.mdpi.com/1424-8220/18/7/2028gold nanoparticlestargeted bacteriaantibody labelingCronobacter sakazakiiimmunogoldplasmon extinction nanoparticle sensingpoly(ethylene glycol) brushspectroscopic detection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohamed A. Aly Konrad J. Domig Wolfgang Kneifel Erik Reimhult |
spellingShingle |
Mohamed A. Aly Konrad J. Domig Wolfgang Kneifel Erik Reimhult Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii Sensors gold nanoparticles targeted bacteria antibody labeling Cronobacter sakazakii immunogold plasmon extinction nanoparticle sensing poly(ethylene glycol) brush spectroscopic detection |
author_facet |
Mohamed A. Aly Konrad J. Domig Wolfgang Kneifel Erik Reimhult |
author_sort |
Mohamed A. Aly |
title |
Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii |
title_short |
Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii |
title_full |
Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii |
title_fullStr |
Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii |
title_full_unstemmed |
Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii |
title_sort |
immunogold nanoparticles for rapid plasmonic detection of c. sakazakii |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2018-06-01 |
description |
Cronobacter sakazakii is a foodborne pathogen that can cause a rare, septicemia, life-threatening meningitis, and necrotizing enterocolitis in infants. In general, standard methods for pathogen detection rely on culture, plating, colony counting and polymerase chain reaction DNA-sequencing for identification, which are time, equipment and skill demanding. Recently, nanoparticle- and surface-based immunoassays have increasingly been explored for pathogen detection. We investigate the functionalization of gold nanoparticles optimized for irreversible and specific binding to C. sakazakii and their use for spectroscopic detection of the pathogen. We demonstrate how 40-nm gold nanoparticles grafted with a poly(ethylene glycol) brush and functionalized with polyclonal antibodies raised against C. sakazakii can be used to specifically target C. sakazakii. The strong extinction peak of the Au nanoparticle plasmon polariton resonance in the optical range is used as a label for detection of the pathogens. Individual binding of the nanoparticles to the C. sakazakii surface is also verified by transmission electron microscopy. We show that a high degree of surface functionalization with anti-C. sakazakii optimizes the detection and leads to a detection limit as low as 10 CFU/mL within 2 h using a simple cuvette-based UV-Vis spectrometric readout that has great potential for further optimization. |
topic |
gold nanoparticles targeted bacteria antibody labeling Cronobacter sakazakii immunogold plasmon extinction nanoparticle sensing poly(ethylene glycol) brush spectroscopic detection |
url |
http://www.mdpi.com/1424-8220/18/7/2028 |
work_keys_str_mv |
AT mohamedaaly immunogoldnanoparticlesforrapidplasmonicdetectionofcsakazakii AT konradjdomig immunogoldnanoparticlesforrapidplasmonicdetectionofcsakazakii AT wolfgangkneifel immunogoldnanoparticlesforrapidplasmonicdetectionofcsakazakii AT erikreimhult immunogoldnanoparticlesforrapidplasmonicdetectionofcsakazakii |
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