Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens.

Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens.

RNAi screening using pooled shRNA libraries is a valuable tool for identifying genetic regulators of biological processes. However, for a successful pooled shRNA screen, it is imperative to thoroughly optimize experimental conditions to obtain reproducible data. Here we performed viability screens w...

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Bibliographic Details
Main Authors: Žaklina Strezoska, Abel Licon, Josh Haimes, Katie Jansen Spayd, Kruti M Patel, Kevin Sullivan, Katarzyna Jastrzebski, Kaylene J Simpson, Devin Leake, Anja van Brabant Smith, Annaleen Vermeulen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870320/pdf/?tool=EBI

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870320/pdf/?tool=EBI

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