Effect of the Compaction and the Size of DNA on the Nuclear Transfer Efficiency after Microinjection in Synchronized Cells

Effect of the Compaction and the Size of DNA on the Nuclear Transfer Efficiency after Microinjection in Synchronized Cells

The nuclear transfer process is one of the critical rate-limiting processes in transgene expression. In the present study, we report on the effect of compaction and the size of the DNA molecule on nuclear transfer efficiency by microinjection. A DNA/protamine complex- or variously-sized naked DNA mo...

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Bibliographic Details
Main Authors: Hidetaka Akita, Dai Kurihara, Marco Schmeer, Martin Schleef, Hideyoshi Harashima
Format: Article
Language:English
Published: MDPI AG 2015-06-01
Series:Pharmaceutics
Subjects:
DNA
nuclear transfer
size
microinjection
Online Access:http://www.mdpi.com/1999-4923/7/2/64

Internet

http://www.mdpi.com/1999-4923/7/2/64

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