A simple alkaline method for decellularizing human amniotic membrane for cell culture.

A simple alkaline method for decellularizing human amniotic membrane for cell culture.

Human amniotic membrane is a standard substratum used to culture limbal epithelial stem cells for transplantation to patients with limbal stem cell deficiency. Various methods were developed to decellularize amniotic membrane, because denuded membrane is poorly immunogenic and better supports repopu...

Full description

Bibliographic Details
Main Authors: Mehrnoosh Saghizadeh, Michael A Winkler, Andrei A Kramerov, David M Hemmati, Chantelle A Ghiam, Slobodan D Dimitrijevich, Dhruv Sareen, Loren Ornelas, Homayon Ghiasi, William J Brunken, Ezra Maguen, Yaron S Rabinowitz, Clive N Svendsen, Katerina Jirsova, Alexander V Ljubimov
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3827346?pdf=render

Internet

http://europepmc.org/articles/PMC3827346?pdf=render

Similar Items