A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease

A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease

Scaling of CRISPR-Cas9 technology in human pluripotent stem cells (hPSCs) represents an important step for modeling complex disease and developing drug screens in human cells. However, variables affecting the scaling efficiency of gene editing in hPSCs remain poorly understood. Here, we report a sta...

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Bibliographic Details
Main Authors: Dane Z. Hazelbaker, Amanda Beccard, Anne M. Bara, Nicole Dabkowski, Angelica Messana, Patrizia Mazzucato, Daisy Lam, Danielle Manning, Kevin Eggan, Lindy E. Barrett
Format: Article
Language:English
Published: Elsevier 2017-10-01
Series:Stem Cell Reports
Subjects:
human pluripotent stem cells
CRISPR-Cas9
psychiatric disease
CRISPR-Cas9 nickase
stem cell-derived human neurons
variant calling
stem cell genomic stability
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671117304137

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http://www.sciencedirect.com/science/article/pii/S2213671117304137

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