Cloning, expression and purification of the α-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis

Cloning, expression and purification of the α-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis

We cloned, expressed, purified, and determined the kinetic constants of the recombinant α-carbonic anhydrase (rec-MgaCA) identified in the mantle tissue of the bivalve Mediterranean mussel, Mytilus galloprovincialis. In metazoans, the α-CA family is largely represented and plays a pivotal role in th...

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Bibliographic Details
Main Authors: Rosa Perfetto, Sonia Del Prete, Daniela Vullo, Vincenzo Carginale, Giovanni Sansone, Carmela M. A. Barone, Mosè Rossi, Fatmah A. S. Alasmary, Sameh M. Osman, Zeid AlOthman, Claudiu T. Supuran, Clemente Capasso
Format: Article
Language:English
Published: Taylor & Francis Group 2017-01-01
Series:Journal of Enzyme Inhibition and Medicinal Chemistry
Subjects:
Carbonic anhydrase
metalloenzymes
α-class enzyme
hydratase activity
mussel
multidomain protein
protonography
bivalve
Online Access:http://dx.doi.org/10.1080/14756366.2017.1353502

Internet

http://dx.doi.org/10.1080/14756366.2017.1353502

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