Sequence determinants of improved CRISPR sgRNA design

Sequence determinants of improved CRISPR sgRNA design

The CRISPR/Cas9 system has revolutionized mammalian somatic cell genetics. Genome-wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated inhibition/activation (CRISPRi/a) are powerful techniques for discovering phenotype-associated gene function. We systematically asses...

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Main Authors: Xu, Han (Author), Xiao, Tengfei (Author), Chen, Chen-Hao (Author), Li, Wei (Author), Meyer, Clifford A. (Author), Wu, Qiu (Author), Wu, Di (Author), Cong, Le (Author), Zhang, Feng (Contributor), Liu, Jun S. (Author), Brown, Myles (Author), Liu, X. Shirley (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Cold Spring Harbor Laboratory Press, 2016-01-11T00:31:23Z.
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