One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

Mice carrying mutations in multiple genes are traditionally generated by sequential recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single mutation. The CRISPR/Cas system has been adapted as an efficient gene-targeting technology with the potential for multip...

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Bibliographic Details
Main Authors: Wang, Haoyi (Author), Yang, Hui (Author), Shivalila, Chikdu Shakti (Contributor), Dawlaty, Meelad M. (Author), Zhang, Feng (Contributor), Jaenisch, Rudolf (Contributor), Cheng, Albert Wu (Author)
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor), Whitehead Institute for Biomedical Research (Contributor), Cheng, Albert W. (Contributor)
Format: Article
Language:English
Published: Cell Press/Elsevier, 2016-06-01T20:53:40Z.
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