Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction

Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction

We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidig...

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Main Authors: Li, Shuqiang (Author), Darmanis, Spyros (Author), Lundberg, Martin (Author), Fredriksson, Simon (Author), Landegren, Ulf (Author), Gallant, Caroline J. (Author), Livak, Kenneth J. (Author), Genshaft, Alex S. (Contributor), Prakadan, Sanjay (Contributor), Ziegler, Carly (Contributor), Hong, Joyce (Contributor), Regev, Aviv (Contributor), Shalek, Alexander K (Contributor)
Other Authors: Massachusetts Institute of Technology. Institute for Medical Engineering & Science (Contributor), Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Ragon Institute of MGH, MIT and Harvard (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor)
Format: Article
Language:English
Published: BioMed Central, 2016-12-12T21:14:31Z.
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