Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

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In recent years, the assay for transposase-accessible chromatin using sequencing (ATAC-Seq) has become a fundamental tool of epigenomic research. However, it is difficult to perform this technique on frozen samples because freezing cells before extracting nuclei can impair nuclear integrity and alte...

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Bibliographic Details
Main Authors: Shelley, Brandon C. (Author), Mandefro, Berhan (Author), Sareen, Dhruv (Author), Svendsen, Clive N. (Author), Milani, Pamela (Contributor), Escalante, Renan A. (Contributor), Patel-Murray, Natasha Leanna (Contributor), Xin, Xiaofeng (Contributor), Adam, Miriam (Contributor), Fraenkel, Ernest (Contributor)
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2017-03-17T17:56:13Z.
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