Modification of proteolytic activity matrix analysis (PrAMA) to measure ADAM10 and ADAM17 sheddase activities in cell and tissue lysates

Modification of proteolytic activity matrix analysis (PrAMA) to measure ADAM10 and ADAM17 sheddase activities in cell and tissue lysates

Increases in expression of ADAM10 and ADAM17 genes and proteins have been evaluated, but not validated as cancer biomarkers. Specific enzyme activities better reflect enzyme cellular functions, and might be better biomarkers than enzyme genes or proteins. However, no high throughput assay is availab...

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Main Authors: Yoneyama, Toshie (Author), Gorry, Michael (Author), Gaither-Davis, Autumn (Author), Lin, Yan (Author), Moss, Marcia L. (Author), Stabile, Laura P. (Author), Herman, James G. (Author), Vujanovic, Nikola L. (Author), Miller, Miles Aaron (Contributor), Griffith, Linda G (Contributor), Lauffenburger, Douglas A (Contributor)
Other Authors: Massachusetts Institute of Technology. Biotechnology Process Engineering Center (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Massachusetts Institute of Technology. Department of Mechanical Engineering (Contributor)
Format: Article
Language:English
Published: Ivyspring International Publisher, 2018-09-04T15:45:07Z.
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