Cellular Barcodes for Efficiently Profiling Single-Cell Secretory Responses by Microengraving

Cellular Barcodes for Efficiently Profiling Single-Cell Secretory Responses by Microengraving

We present a method that uses fluorescent cellular barcodes to increase the number of unique samples that can be analyzed simultaneously by microengraving, a nanowell array-based technique for quantifying the secretory responses of thousands of single cells in parallel. Using n different fluorescent...

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Main Authors: Gierahn, Todd Michael (Contributor), Forcier, Talitha L. (Contributor), Brefo, Mavis S. (Contributor), Yamanaka, Yvonne J. (Author), Szeto, Gregory (Author), Arnold, Kelly B. (Author), Lauffenburger, Douglas A (Author), Irvine, Darrell J (Author), Love, John C (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Massachusetts Institute of Technology. Department of Materials Science and Engineering (Contributor), Ragon Institute of MGH, MIT and Harvard (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor), Love, J. Christopher (Contributor), Yamanaka, Yvonne Joy (Contributor), Szeto, Gregory Lee (Contributor), Benedict, Kelly (Contributor), Lauffenburger, Douglas A. (Contributor), Irvine, Darrell J. (Contributor)
Format: Article
Language:English
Published: American Chemical Society, 2013-07-26T15:15:15Z.
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