Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformat...

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Main Authors: Trifonova, Radiana (Author), Jhunjhunwala, Siddharth (Contributor), Hartoularos, George C. (Contributor), Eyerman, Alexandra T. (Contributor), Lytton-Jean, Abigail K. R. (Contributor), Angin, Mathieu (Author), Sharma, Siddhartha (Author), Poceviciute, Roberta (Contributor), Mao, Shirley (Contributor), Heimann, Megan (Contributor), Liu, Sophia (Contributor), Talkar, Tanya (Contributor), Khan, Omar F. (Contributor), Addo, Marylyn M. (Author), von Andrian, Ulrich H. (Author), Langer, Robert (Contributor), Lieberman, Judy (Author), Jensen, Klavs F. (Contributor), Sharei, Armon Reza (Contributor), Anderson, Daniel Griffith (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Ragon Institute of MGH, MIT and Harvard (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor)
Format: Article
Language:English
Published: Public Library of Science, 2015-05-29T15:01:49Z.
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