Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 95 === Abstract
Chitin is the mainly constitution of invertebrates and the cell wall of fungus. The different degree of N-acetylation(DA) can influence the properties of chitin and chitosan, so to determine DA is important.
Using the improved HPLC A and B can determine chitin and chitosan with DA range from 25 to 85%. Improved conditions of acid hydrolysis which increase the content of sulfuric acid from 0.5 mL to 1.5 mL, decrease the temperature from 155℃ to 110℃ and time of acid hydrolysis from 1 hrs to 30-40 mins, it can reduce sugar caramelization and the time of samples preparation.
The improved HPLC A and B is more accurate and more precise than other ways, such as IR spectroscopy、UV spectroscopy etc ; therefore using IR spectroscopy determine the DA of samples, we can use the improved HPLC A to take place of NMR spectroscopy to build the calibration curves which become a standard of IR spectroscopy.
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