Safety assessment of two new Lactobacillus strains as probiotic for human using a rat model

• Main Authors: Faseleh Jahromi, M. (Author), Ho, Y.W (Author), Kalavathy, R. (Author), Liang, J.B (Author), Shokryazdan, P. (Author), Sieo, C.C (Author)
• Format: Article
• Language: English
• Published: Public Library of Science 2016
• Subjects: acute toxicity; animal; animal experiment; animal model; animal tissue; Animals; Article; bacterial enzyme; bacterial growth; bacterial strain; bacterium identification; beta glucosidase; beta glucuronidase; biochemistry; blood analysis; blood component; body weight; Body Weight; cecum; Cecum; controlled study; drug effect; enzyme activity; enzyme assay; enzymology; feeding behavior; Feeding Behavior; female; Female; food intake; growth rate; histopathology; human; Humans; intestine flora; Lactobacillus; Lactobacillus buchneri; Lactobacillus fermentum; male; Male; microbiology; Models, Animal; molecular dynamics; nonhuman; oral toxicity; organ weight; probiotic agent; Probiotics; rat; Rats; Rats, Sprague-Dawley; risk assessment; species difference; Species Specificity; Sprague Dawley rat; toxicity; toxicity testing; Toxicity Tests
• Online Access: View Fulltext in Publisher; View in Scopus

 Two previously isolated Lactobacillus strains (L. fermentum HM3 from human milk and L. buchneri FD2 from fermented dates), intended as probiotic for human, were assessed for their safety using acute and subacute oral toxicity tests in rats. In addition, their effects on cecal microflora and harmful bacterial enzymes (β-glucuronidase and β-glucosidase) of the tested animals were also determined. The results showed that L. buchneri FD2, L. fermentum HM3, or a mixture of them were safe up to a level of 1010 CFU/kg BW/day in a 14-day or 28-day treatment period. Both strains were well tolerated and there were no observed adverse effects on growth, feed consumption, cellular blood components and vital organs of the treated animals. The Lactobacillus strains were also able to reduce harmful intestinal bacterial enzymes, and decrease pathogenic bacterial populations while increasing beneficial bacterial populations. These results suggest that the two Lactobacillus strains are safe and could be potential probiotic for human. © 2016 Shokryazdan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.