Increasing quality, throughput and speed of sample preparation for strand-specific messenger RNA sequencing

Increasing quality, throughput and speed of sample preparation for strand-specific messenger RNA sequencing

Abstract Background RNA-Sequencing (RNA-seq) is now commonly used to reveal quantitative spatiotemporal snapshots of the transcriptome, the structures of transcripts (splice variants and fusions) and landscapes of expressed mutations. However, standard approaches for library construction typically r...

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Bibliographic Details
Main Authors: Simon Haile, Richard D. Corbett, Tina MacLeod, Steve Bilobram, Duane Smailus, Philip Tsao, Heather Kirk, Helen McDonald, Pawan Pandoh, Miruna Bala, Martin Hirst, Diane Miller, Richard A. Moore, Andrew J. Mungall, Jacquie Schein, Robin J. Coope, Yussanne Ma, Yongjun Zhao, Rob A. Holt, Steven J. Jones, Marco A. Marra
Format: Article
Language:English
Published: BMC 2017-07-01
Series:BMC Genomics
Subjects:
Ampure XP magnetic beads
Next-generation sequencing
Library construction
Strand-specific
dUTP
Uracil DNA N-Glycosylase
Online Access:http://link.springer.com/article/10.1186/s12864-017-3900-6

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http://link.springer.com/article/10.1186/s12864-017-3900-6

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