A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

Cells employ different repair pathways to repair DNA double strand breaks. Here, the authors develop a CRISPR/Cas9-dependent method to study choices in DNA repair called the Color Assay Tracing-Repair (CAT-R) which simultaneously measure outcomes of DSB repair via end-protection and end-resection pa...

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Bibliographic Details
Main Authors: Paris Roidos, Stephanie Sungalee, Salvatore Benfatto, Özdemirhan Serçin, Adrian M. Stütz, Amir Abdollahi, Jan Mauer, Frank T. Zenke, Jan O. Korbel, Balca R. Mardin
Format: Article
Language:English
Published: Nature Publishing Group 2020-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-17962-3

Internet

https://doi.org/10.1038/s41467-020-17962-3

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