A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

Cells employ different repair pathways to repair DNA double strand breaks. Here, the authors develop a CRISPR/Cas9-dependent method to study choices in DNA repair called the Color Assay Tracing-Repair (CAT-R) which simultaneously measure outcomes of DSB repair via end-protection and end-resection pa...

Full description

Bibliographic Details
Main Authors: Paris Roidos, Stephanie Sungalee, Salvatore Benfatto, Özdemirhan Serçin, Adrian M. Stütz, Amir Abdollahi, Jan Mauer, Frank T. Zenke, Jan O. Korbel, Balca R. Mardin
Format: Article
Language:English
Published: Nature Publishing Group 2020-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-17962-3
id doaj-229117b82514431a854b76cda0b7c54e
record_format Article
spelling doaj-229117b82514431a854b76cda0b7c54e2021-08-15T11:40:47ZengNature Publishing GroupNature Communications2041-17232020-08-0111111510.1038/s41467-020-17962-3A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choiceParis Roidos0Stephanie Sungalee1Salvatore Benfatto2Özdemirhan Serçin3Adrian M. Stütz4Amir Abdollahi5Jan Mauer6Frank T. Zenke7Jan O. Korbel8Balca R. Mardin9BioMed X Institute (GmbH), Im Neuenheimer Feld 583European Molecular Biology Laboratory, Genome Biology UnitBioMed X Institute (GmbH), Im Neuenheimer Feld 583BioMed X Institute (GmbH), Im Neuenheimer Feld 583European Molecular Biology Laboratory, Genome Biology UnitDivision of Molecular and Translational Radiation Oncology, National Centre for Tumour Diseases (NCT), Heidelberg University HospitalBioMed X Institute (GmbH), Im Neuenheimer Feld 583Translational Innovation Platform Oncology, Merck KGaAEuropean Molecular Biology Laboratory, Genome Biology UnitBioMed X Institute (GmbH), Im Neuenheimer Feld 583Cells employ different repair pathways to repair DNA double strand breaks. Here, the authors develop a CRISPR/Cas9-dependent method to study choices in DNA repair called the Color Assay Tracing-Repair (CAT-R) which simultaneously measure outcomes of DSB repair via end-protection and end-resection pathways.https://doi.org/10.1038/s41467-020-17962-3
collection DOAJ
language English
format Article
sources DOAJ
author Paris Roidos
Stephanie Sungalee
Salvatore Benfatto
Özdemirhan Serçin
Adrian M. Stütz
Amir Abdollahi
Jan Mauer
Frank T. Zenke
Jan O. Korbel
Balca R. Mardin
spellingShingle Paris Roidos
Stephanie Sungalee
Salvatore Benfatto
Özdemirhan Serçin
Adrian M. Stütz
Amir Abdollahi
Jan Mauer
Frank T. Zenke
Jan O. Korbel
Balca R. Mardin
A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
Nature Communications
author_facet Paris Roidos
Stephanie Sungalee
Salvatore Benfatto
Özdemirhan Serçin
Adrian M. Stütz
Amir Abdollahi
Jan Mauer
Frank T. Zenke
Jan O. Korbel
Balca R. Mardin
author_sort Paris Roidos
title A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_short A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_full A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_fullStr A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_full_unstemmed A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_sort scalable crispr/cas9-based fluorescent reporter assay to study dna double-strand break repair choice
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-08-01
description Cells employ different repair pathways to repair DNA double strand breaks. Here, the authors develop a CRISPR/Cas9-dependent method to study choices in DNA repair called the Color Assay Tracing-Repair (CAT-R) which simultaneously measure outcomes of DSB repair via end-protection and end-resection pathways.
url https://doi.org/10.1038/s41467-020-17962-3
work_keys_str_mv AT parisroidos ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT stephaniesungalee ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT salvatorebenfatto ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT ozdemirhansercin ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT adrianmstutz ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT amirabdollahi ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT janmauer ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT franktzenke ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT janokorbel ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT balcarmardin ascalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT parisroidos scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT stephaniesungalee scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT salvatorebenfatto scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT ozdemirhansercin scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT adrianmstutz scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT amirabdollahi scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT janmauer scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT franktzenke scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT janokorbel scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
AT balcarmardin scalablecrisprcas9basedfluorescentreporterassaytostudydnadoublestrandbreakrepairchoice
_version_ 1721206495619579904

Similar Items