Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks
DNA replication errors that persist as mismatch mutations make up the molecular fingerprint of mismatch repair (MMR)-deficient tumors and convey them with resistance to standard therapy. Using whole-genome and whole-exome sequencing, we here confirm an MMR-deficient mutation signature that is distin...
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doaj-3686d089d2d84213816650f5cb0bc4ec2021-05-04T23:24:20ZengeLife Sciences Publications LtdeLife2050-084X2014-08-01310.7554/eLife.02725Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaksHui Zhao0Bernard Thienpont1https://orcid.org/0000-0002-8772-6845Betül Tuba Yesilyurt2Matthieu Moisse3https://orcid.org/0000-0001-8880-9311Joke Reumers4Lieve Coenegrachts5Xavier Sagaert6Stefanie Schrauwen7Dominiek Smeets8Gert Matthijs9Stein Aerts10Jan Cools11Alex Metcalf12Amanda Spurdle13ANECS14Frederic Amant15Diether Lambrechts16VIB Vesalius Research Center, KU Leuven, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, BelgiumVIB Vesalius Research Center, KU Leuven, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, BelgiumVIB Vesalius Research Center, KU Leuven, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, BelgiumVIB Vesalius Research Center, KU Leuven, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, BelgiumVIB Vesalius Research Center, KU Leuven, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, BelgiumDivision of Gynaecologic Oncology, Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, BelgiumDivision of Pathology, University Hospital Gasthuisberg, Leuven, BelgiumDivision of Gynaecologic Oncology, Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, BelgiumVIB Vesalius Research Center, KU Leuven, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, BelgiumDepartment of Human Genetics, KU Leuven, Leuven, BelgiumDepartment of Human Genetics, KU Leuven, Leuven, BelgiumDepartment of Human Genetics, KU Leuven, Leuven, Belgium; VIB Center for the Biology of Disease, KU Leuven, Leuven, BelgiumDivision of Genetics and Computational Biology, Queensland Institute of Medical Research, Brisbane, AustraliaDivision of Genetics and Computational Biology, Queensland Institute of Medical Research, Brisbane, AustraliaThe Australian National Endometrial Cancer Study, PO Royal Brisbane Hospital, Brisbane, AustraliaDivision of Gynaecologic Oncology, Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, BelgiumVIB Vesalius Research Center, KU Leuven, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, BelgiumDNA replication errors that persist as mismatch mutations make up the molecular fingerprint of mismatch repair (MMR)-deficient tumors and convey them with resistance to standard therapy. Using whole-genome and whole-exome sequencing, we here confirm an MMR-deficient mutation signature that is distinct from other tumor genomes, but surprisingly similar to germ-line DNA, indicating that a substantial fraction of human genetic variation arises through mutations escaping MMR. Moreover, we identify a large set of recurrent indels that may serve to detect microsatellite instability (MSI). Indeed, using endometrial tumors with immunohistochemically proven MMR deficiency, we optimize a novel marker set capable of detecting MSI and show it to have greater specificity and selectivity than standard MSI tests. Additionally, we show that recurrent indels are enriched for the ‘DNA double-strand break repair by homologous recombination’ pathway. Consequently, DSB repair is reduced in MMR-deficient tumors, triggering a dose-dependent sensitivity of MMR-deficient tumor cultures to DSB inducers.https://elifesciences.org/articles/02725whole-genome sequencingmismatch repair deficiencymutation patternMSIDNA double-strand breakDSB inducer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui Zhao Bernard Thienpont Betül Tuba Yesilyurt Matthieu Moisse Joke Reumers Lieve Coenegrachts Xavier Sagaert Stefanie Schrauwen Dominiek Smeets Gert Matthijs Stein Aerts Jan Cools Alex Metcalf Amanda Spurdle ANECS Frederic Amant Diether Lambrechts |
spellingShingle |
Hui Zhao Bernard Thienpont Betül Tuba Yesilyurt Matthieu Moisse Joke Reumers Lieve Coenegrachts Xavier Sagaert Stefanie Schrauwen Dominiek Smeets Gert Matthijs Stein Aerts Jan Cools Alex Metcalf Amanda Spurdle ANECS Frederic Amant Diether Lambrechts Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks eLife whole-genome sequencing mismatch repair deficiency mutation pattern MSI DNA double-strand break DSB inducer |
author_facet |
Hui Zhao Bernard Thienpont Betül Tuba Yesilyurt Matthieu Moisse Joke Reumers Lieve Coenegrachts Xavier Sagaert Stefanie Schrauwen Dominiek Smeets Gert Matthijs Stein Aerts Jan Cools Alex Metcalf Amanda Spurdle ANECS Frederic Amant Diether Lambrechts |
author_sort |
Hui Zhao |
title |
Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks |
title_short |
Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks |
title_full |
Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks |
title_fullStr |
Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks |
title_full_unstemmed |
Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks |
title_sort |
mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to dna double-strand breaks |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2014-08-01 |
description |
DNA replication errors that persist as mismatch mutations make up the molecular fingerprint of mismatch repair (MMR)-deficient tumors and convey them with resistance to standard therapy. Using whole-genome and whole-exome sequencing, we here confirm an MMR-deficient mutation signature that is distinct from other tumor genomes, but surprisingly similar to germ-line DNA, indicating that a substantial fraction of human genetic variation arises through mutations escaping MMR. Moreover, we identify a large set of recurrent indels that may serve to detect microsatellite instability (MSI). Indeed, using endometrial tumors with immunohistochemically proven MMR deficiency, we optimize a novel marker set capable of detecting MSI and show it to have greater specificity and selectivity than standard MSI tests. Additionally, we show that recurrent indels are enriched for the ‘DNA double-strand break repair by homologous recombination’ pathway. Consequently, DSB repair is reduced in MMR-deficient tumors, triggering a dose-dependent sensitivity of MMR-deficient tumor cultures to DSB inducers. |
topic |
whole-genome sequencing mismatch repair deficiency mutation pattern MSI DNA double-strand break DSB inducer |
url |
https://elifesciences.org/articles/02725 |
work_keys_str_mv |
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