Assay Format as a Critical Success Factor for Identification of Novel Inhibitor Chemotypes of Tissue-Nonspecific Alkaline Phosphatase from High-Throughput Screening

Assay Format as a Critical Success Factor for Identification of Novel Inhibitor Chemotypes of Tissue-Nonspecific Alkaline Phosphatase from High-Throughput Screening

The tissue-nonspecific alkaline phosphatase (TNAP) isozyme is centrally involved in the control of normal skeletal mineralization and pathophysiological abnormalities that lead to disease states such as hypophosphatasia, osteoarthritis, ankylosis and vascular calcification. TNAP acts in concert with...

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Bibliographic Details
Main Authors: José Luis Millán, Eduard Sergienko, Thomas D.Y. Chung
Format: Article
Language:English
Published: MDPI AG 2010-04-01
Series:Molecules
Subjects:
diethanolamine (DEA)
absorption spectroscopy
luminescence
high throughput screening
CDP-Star®
Molecular Libraries
tissue-nonspecific alkaline phosphatase
alkaline phosphatase
chemical library
para-nitrophenylphosphate
Online Access:http://www.mdpi.com/1420-3049/15/5/3010/

Internet

http://www.mdpi.com/1420-3049/15/5/3010/

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