Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

Current advances in producing genetically modified mice using genome-editing technologies have indicated the need for improvement of limiting factors including zygote collection for microinjection and their cryopreservation. Recently, we developed a novel superovulation technique using inhibin antis...

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Bibliographic Details
Main Authors: Yoshiko Nakagawa, Tetsushi Sakuma, Norihisa Nishimichi, Yasuyuki Yokosaki, Noriyuki Yanaka, Toru Takeo, Naomi Nakagata, Takashi Yamamoto
Format: Article
Language:English
Published: The Company of Biologists 2016-08-01
Series:Biology Open
Subjects:
Knockout
Floxed
CRISPR-Cas9
Zygote
Ultra-superovulation
Inhibin antiserum and equine chorionic gonadotropin (IASe)
Online Access:http://bio.biologists.org/content/5/8/1142

Internet

http://bio.biologists.org/content/5/8/1142

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