Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells

Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells

Selection of single antigen-specific B cells to identify their expressed antibodies is of considerable interest for evaluating human immune responses. Here, we present a method to identify single antibody-expressing cells using antigen-conjugated fluorescent beads. To establish this, we selected Fol...

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Bibliographic Details
Main Authors: Isabel Correa, Kristina M. Ilieva, Silvia Crescioli, Sara Lombardi, Mariangela Figini, Anthony Cheung, James F. Spicer, Andrew N. J. Tutt, Frank O. Nestle, Panagiotis Karagiannis, Katie E. Lacy, Sophia N. Karagiannis
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-03-01
Series:Frontiers in Immunology
Subjects:
B cell
humoral immune response
single cell sorting
fluorescent bead
human antibodies
antibody expression
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2018.00493/full

Internet

http://journal.frontiersin.org/article/10.3389/fimmu.2018.00493/full

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