An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions

An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions

Summary: Here, we describe a xeno-free, feeder-free, and chemically defined protocol for the generation of ventral midbrain dopaminergic (vmDA) progenitors from human pluripotent stem cells (hPSCs). This simple-to-follow protocol results in high yields of cryopreservable dopamine neurons across mult...

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Bibliographic Details
Main Authors: Carlos W. Gantner, Agustín Cota-Coronado, Lachlan H. Thompson, Clare L. Parish
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300526

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http://www.sciencedirect.com/science/article/pii/S2666166720300526

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