Cas9 gRNA engineering for genome editing, activation and repression

Cas9 gRNA engineering for genome editing, activation and repression

We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits wit...

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Bibliographic Details
Main Authors: Chavez, Alejandro (Author), Tuttle, Marcelle (Author), Chari, Raj (Author), Ter-Ovanesyan, Dmitry (Author), Qian, Jason (Author), Pruitt, Benjamin W (Author), Buchthal, Joanna (Author), Church, George (Author), Kiani, Samira (Contributor), Hall, Richard N. (Contributor), Beal, Jacob S (Contributor), Vora, Suhani Deepak (Contributor), Kowal, Emma J. (Contributor), Ebrahimkhani, Mohammad Reza (Contributor), Collins, James J. (Contributor), Weiss, Ron (Contributor)
Other Authors: MIT Synthetic Biology Center (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2017-04-06T19:57:41Z.
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