Cas9 gRNA engineering for genome editing, activation and repression
We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits wit...
Main Authors: | , , , , , , , , , , , , , , , |
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Other Authors: | , |
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group,
2017-04-06T19:57:41Z.
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Subjects: | |
Online Access: | Get fulltext |
Summary: | We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein. National Human Genome Research Institute (U.S.) (P50 HG005550) United States. Department of Energy (DE-FG02-02ER63445) Wyss Institute for Biologically Inspired Engineering United States. Army Research Office (DARPA W911NF-11-2-0054) National Science Foundation (U.S.) United States. National Institutes of Health (5R01CA155320-04) United States. National Institutes of Health (P50 GM098792) National Cancer Institute (U.S.) (5T32CA009216-34) Massachusetts Institute of Technology. Department of Biological Engineering Harvard Medical School. Department of Genetics Defense Threat Reduction Agency (DTRA) (HDTRA1-14-1-0006) |
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