An enhanced CRISPR repressor for targeted mammalian gene regulation

An enhanced CRISPR repressor for targeted mammalian gene regulation

The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility. Here we describe an improved Cas9 repressor based on the C-terminal fusion of a rationally designed bipartite repressor domain, KRAB-...

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Main Authors: Yeo, Nan Cher (Author), Chavez, Alejandro (Author), Lance-Byrne, Alissa (Author), Chan, Yingleong (Author), Menn, David (Author), Milanova, Denitsa (Author), Kuo, Chih-Chung (Author), Guo, Xiaoge (Author), Sharma, Sumana (Author), Tung, Angela (Author), Cecchi, Ryan J. (Author), Tuttle, Marcelle (Author), Pradhan, Swechchha (Author), Lim, Elaine T. (Author), Davidsohn, Noah (Author), Ebrahimkhani, Mo R. (Author), Lewis, Nathan E. (Author), Kiani, Samira (Author), Church, George M. (Author), Collins, James J. (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Collins, James J (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2019-02-13T15:53:38Z.
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