Highly efficient Cas9-mediated transcriptional programming

Highly efficient Cas9-mediated transcriptional programming

The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR)...

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Main Authors: Chavez, Alejandro (Author), Scheiman, Jonathan (Author), Tuttle, Marcelle (Author), Lin, Shuailiang (Author), Kiani, Samira (Contributor), Ter-Ovanesyan, Dmitry (Author), Davidsohn, Noah (Author), Perrimon, Norbert (Author), Weiss, Ron (Contributor), Aach, John (Author), Vora, Suhani Deepak (Contributor), Pruitt, Benjamin W. (Author), Iyer, Eswar P. R. (Author), Guzman, Christopher D. (Author), Wiegand, Daniel J. (Author), Braff, Jonathan L. (Author), Housden, Benjamin E. (Author), Collins, James J. (Contributor), Church, George M. (Author)
Other Authors: Institute for Medical Engineering and Science (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Massachusetts Institute of Technology. Synthetic Biology Center (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2015-10-30T17:46:23Z.
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