Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool.

by Katherine F Donovan, Mudra Hegde, Meagan Sullender, Emma W Vaimberg, Cory M Johannessen, David E Root, John G Doench
Published 2017-01-01

Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

by Joseph Rosenbluh, Han Xu, William Harrington, Stanley Gill, Xiaoxing Wang, Francisca Vazquez, David E. Root, Aviad Tsherniak, William C. Hahn
Published 2017-05-01

Aire-dependent genes undergo Clp1-mediated 3’UTR shortening associated with higher transcript stability in the thymus

by Clotilde Guyon, Nada Jmari, Francine Padonou, Yen-Chin Li, Olga Ucar, Noriyuki Fujikado, Fanny Coulpier, Christophe Blanchet, David E Root, Matthieu Giraud
Published 2020-04-01

High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.

by Thomas J F Nieland, David J Logan, Jessica Saulnier, Daniel Lam, Caroline Johnson, David E Root, Anne E Carpenter, Bernardo L Sabatini
Published 2014-01-01

Morphological Profiles of RNAi-Induced Gene Knockdown Are Highly Reproducible but Dominated by Seed Effects.

by Shantanu Singh, Xiaoyun Wu, Vebjorn Ljosa, Mark-Anthony Bray, Federica Piccioni, David E Root, John G Doench, Jesse S Boehm, Anne E Carpenter
Published 2015-01-01

Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map.

by Ian Smith, Peyton G Greenside, Ted Natoli, David L Lahr, David Wadden, Itay Tirosh, Rajiv Narayan, David E Root, Todd R Golub, Aravind Subramanian, John G Doench
Published 2017-11-01

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

by Kendall R. Sanson, Ruth E. Hanna, Mudra Hegde, Katherine F. Donovan, Christine Strand, Meagan E. Sullender, Emma W. Vaimberg, Amy Goodale, David E. Root, Federica Piccioni, John G. Doench
Published 2018-12-01

The master regulator of the cellular stress response (HSF1) is critical for orthopoxvirus infection.

by Claire Marie Filone, Ignacio S Caballero, Ken Dower, Marc L Mendillo, Glenn S Cowley, Sandro Santagata, Daniel K Rozelle, Judy Yen, Kathleen H Rubins, Nir Hacohen, David E Root, Lisa E Hensley, John Connor
Published 2014-02-01

CK1epsilon is required for breast cancers dependent on beta-catenin activity.

by So Young Kim, Ian F Dunn, Ron Firestein, Piyush Gupta, Leslie Wardwell, Kara Repich, Anna C Schinzel, Ben Wittner, Serena J Silver, David E Root, Jesse S Boehm, Sridhar Ramaswamy, Eric S Lander, William C Hahn
Published 2010-02-01

Correction: Corrigendum: PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA

by Tovah A. Day, Jacob V. Layer, J. Patrick Cleary, Srijoy Guha, Kristen E. Stevenson, Trevor Tivey, Sunhee Kim, Anna C Schinzel, Francesca Izzo, John Doench, David E. Root, William C. Hahn, Brendan D. Price, David M. Weinstock
Published 2017-06-01

PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA

by Tovah A. Day, Jacob V. Layer, J. Patrick Cleary, Srijoy Guha, Kristen E. Stevenson, Trevor Tivey, Sunhee Kim, Anna C. Schinzel, Francesca Izzo, John Doench, David E. Root, William C. Hahn, Brendan D. Price, David M. Weinstock
Published 2017-04-01

Phenotypic Characterization of a Comprehensive Set of MAPK1/ERK2 Missense Mutants

by Lisa Brenan, Aleksandr Andreev, Ofir Cohen, Sasha Pantel, Atanas Kamburov, Davide Cacchiarelli, Nicole S. Persky, Cong Zhu, Mukta Bagul, Eva M. Goetz, Alex B. Burgin, Levi A. Garraway, Gad Getz, Tarjei S. Mikkelsen, Federica Piccioni, David E. Root, Cory M. Johannessen
Published 2016-10-01

Improved estimation of cancer dependencies from large-scale RNAi screens using model-based normalization and data integration

by James M. McFarland, Zandra V. Ho, Guillaume Kugener, Joshua M. Dempster, Phillip G. Montgomery, Jordan G. Bryan, John M. Krill-Burger, Thomas M. Green, Francisca Vazquez, Jesse S. Boehm, Todd R. Golub, William C. Hahn, David E. Root, Aviad Tsherniak
Published 2018-11-01

Regulation of Cellular Heterogeneity and Rates of Symmetric and Asymmetric Divisions in Triple-Negative Breast Cancer

by Roy Z. Granit, Hadas Masury, Reba Condiotti, Yaakov Fixler, Yael Gabai, Tzofia Glikman, Simona Dalin, Eitan Winter, Yuval Nevo, Einat Carmon, Tamar Sella, Amir Sonnenblick, Tamar Peretz, Ulrich Lehmann, Keren Paz, Federica Piccioni, Aviv Regev, David E. Root, Ittai Ben-Porath
Published 2018-09-01

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis

by Satish K Nandakumar, Sean K McFarland, Laura M Mateyka, Caleb A Lareau, Jacob C Ulirsch, Leif S Ludwig, Gaurav Agarwal, Jesse M Engreitz, Bartlomiej Przychodzen, Marie McConkey, Glenn S Cowley, John G Doench, Jaroslaw P Maciejewski, Benjamin L Ebert, David E Root, Vijay G Sankaran
Published 2019-05-01

Synergistic interactions with PI3K inhibition that induce apoptosis

by Yaara Zwang, Oliver Jonas, Casandra Chen, Mikael L Rinne, John G Doench, Federica Piccioni, Li Tan, Hai-Tsang Huang, Jinhua Wang, Young Jin Ham, Joyce O'Connell, Patrick Bhola, Mihir Doshi, Matthew Whitman, Michael Cima, Anthony Letai, David E Root, Robert S Langer, Nathanael Gray, William C Hahn
Published 2017-05-01

A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor

by Carla Eller, Laura Heydmann, Che C. Colpitts, Houssein El Saghire, Federica Piccioni, Frank Jühling, Karim Majzoub, Caroline Pons, Charlotte Bach, Julie Lucifora, Joachim Lupberger, Michael Nassal, Glenn S. Cowley, Naoto Fujiwara, Sen-Yung Hsieh, Yujin Hoshida, Emanuele Felli, Patrick Pessaux, Camille Sureau, Catherine Schuster, David E. Root, Eloi R. Verrier, Thomas F. Baumert
Published 2020-06-01

An alternative splicing switch in FLNB promotes the mesenchymal cell state in human breast cancer

by Ji Li, Peter S Choi, Christine L Chaffer, Katherine Labella, Justin H Hwang, Andrew O Giacomelli, Jong Wook Kim, Nina Ilic, John G Doench, Seav Huong Ly, Chao Dai, Kimberly Hagel, Andrew L Hong, Ole Gjoerup, Shom Goel, Jennifer Y Ge, David E Root, Jean J Zhao, Angela N Brooks, Robert A Weinberg, William C Hahn
Published 2018-07-01

SMAD4 represses FOSL1 expression and pancreatic cancer metastatic colonization

by Chao Dai, Jonathan P. Rennhack, Taylor E. Arnoff, Maneesha Thaker, Scott T. Younger, John G. Doench, August Yue Huang, Annan Yang, Andrew J. Aguirre, Belinda Wang, Evan Mun, Joyce T. O’Connell, Ying Huang, Katherine Labella, Jessica A. Talamas, Ji Li, Nina Ilic, Justin Hwang, Andrew L. Hong, Andrew O. Giacomelli, Ole Gjoerup, David E. Root, William C. Hahn
Published 2021-07-01

Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets

by Joshua M. Dempster, Clare Pacini, Sasha Pantel, Fiona M. Behan, Thomas Green, John Krill-Burger, Charlotte M. Beaver, Scott T. Younger, Victor Zhivich, Hanna Najgebauer, Felicity Allen, Emanuel Gonçalves, Rebecca Shepherd, John G. Doench, Kosuke Yusa, Francisca Vazquez, Leopold Parts, Jesse S. Boehm, Todd R. Golub, William C. Hahn, David E. Root, Mathew J. Garnett, Aviad Tsherniak, Francesco Iorio
Published 2019-12-01